爱威斯产品gelsolin试剂盒助力科学家发表国际科学期刊
美国爱威斯生物产品 human plasma gelsolin elisa kit SK00384-01产品助力科学家发表国际科学期刊。
PLoS One. 2019; 14(8): e0221509.
Published online 2019 Aug 22. doi: 10.1371/journal.pone.0221509
A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia
Sandra Isabel Calderon-Rodríguez, Investigation, Methodology, Validation, Visualization, Writing – original draft,1María Carolina Sanabria-Salas, Formal analysis,1,2 and Adriana Umaña-Perez, Conceptualization, Funding acquisition, Writing – review & editing1,*
Abstract
Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Owing to the incorporation of risk-adapted therapy and the arrival of new directed agents, the cure rate and survival of patients with ALL have improved dramatically, get near to 90%. In Latin American countries, the mortality rates of ALL are high, for example in Colombia, during the last decade, ALL has been the most prevalent cancer among children between 0–14 years of age. In the face of this public health problem and coupled with the fact that the knowledge of the proteome of the child population is little, our investigation proposes the study of the plasma proteome of Colombian children diagnosed with B-cell ALL (B-ALL) to determine potential disease markers that could reflect processes altered by the presence of the disease or in response to it. A proteomic study by LC-MS/MS and quantification by label-free methods were performed in search of proteins differentially expressed between healthy children and those diagnosed with B-ALL. We quantified a total of 472 proteins in depleted blood plasma, and 25 of these proteins were differentially expressed (fold change >2, Bonferroni-adjusted P-values <0.05). Plasma Aggrecan core protein, alpha-2-HS-glycoprotein, coagulation factor XIII A chain and gelsolin protein were examined by ELISA assay and compared to shotgun proteomics results. Our data provide new information on the plasma proteome of Colombian children. Additionally, these proteins may also have certain potential as illness markers or as therapeutic targets in subsequent investigations.
Citations:
Meshach Asare-Werehene, et al. Longitudinal profiles of plasma gelsolin, cytokines and antibody expression predict COVID-19 severity and hospitalization outcomes. Front. Immunol., 06 September 2022. Sec. Cytokines and Soluble Mediators in Immunity. https://doi.org/10.3389/fimmu.2022.1011084
Meshach Asare-Werehene, et al. Plasma Gelsolin Confers Chemoresistance in Ovarian Cancer by Resetting the Relative Abundance and Function of Macrophage Subtypes. Cancers 2022, 14(4), 1039; https://doi.org/10.3390/cancers14041039
Meshach Asare-Werehene, et al. The exosome-mediated autocrine and paracrine actions of plasma gelsolin in ovarian cancer chemoresistance. Oncogene (2020) 39:1600–1616
Meshach Asare-Werehene, Laudine Communal, et al. "Pre-operative Circulating Plasma Gelsolin Predicts Residual Disease." Scientific Reports 9, Article Number: 13924 (2019). https://www.nature.com/articles/s41598-019-50436-1
Calderon-Rodríguez SI, Sanabria-Salas MC, Umaña-Perez A (2019) A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia. PLoS ONE 14(8): e0221509. https://doi.org/10.1371/journal.pone.0221509
Gungor, Hatice Eke et al. “The plasma gelsolin levels in atopic dermatitis: Effect of atopy and disease severity.” Allergologia et immunopathologia 44 3 (2016): 221-5.
Pierredon, Sandra & Ribaux, Pascale & Tille. et al. (2017). Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight. Oncology Letters. 13. 10.3892/ol.2017.6096.
Suhara, Kozo et al. Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern Allergology International, Volume 65, Issue 1, 88 - 95
Meshach Asare-Werehene, Laudine Communal, et al. "Pre-operative Circulating Plasma Gelsolin Predicts Residual Disease." Scientific Reports 9, Article Number: 13924 (2019). https://www.nature.com/articles/s41598-019-50436-1
Features
Size: 96 T
Standard Range: 390-50000 pg/ml
Sensitivity: 62.5 pg/mL
Calibration: rh plasma Gelsolin Rec.
Specificity: Human Plasma GelsolinSample Type: plasma, serum
Sample Dilution: 10000~20000
Intra-CV: 4-6%
Inter-CV: 4-9%
