血管紧张素转化酶2 (ACE2)（人）(HEK293)

产品名称：血管紧张素转化酶2 (ACE2)（人）(HEK293)

英文名称：Angiotensin-Converting Enzyme 2 (ACE2) (Human) (HEK293)

产品编号：00707-01-100

规格：100 ug

Feature:

• Soluble ACE2 (Human) His Tagged (HEK293) at 20 ug per vial.

• Tag: His Tag on its C-terminus

• Expression: HEK293 cells animal free culture

• Purity:> 95%

• MW: 110-120 KD (Due to Glycoylation)

• Size: 50 ug

• Tested by anti His Tagged Protein Monoclonal Antibody on RBD-S1 Spike Protein pre-coated microplates.

• Tested by Biotinylated RBD His Tag and Streptavidin-HRP on human ACE2 His Tag Pre-coated Microplates.

• The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH,
  is >1000 pmols/min/μg.

• Endotoxin<1.0 EU per μg of the protein as determined by the LAL method

References:

• Gheblawi M., et al. Angiotensin-Converting Enzyme 2: SARS-CoV-2 Receptor and Regulator of the Renin-Angiotensin System. Circulation Research. 2020;126:1456–1474

Features

•  

  • Gene ID: NM_021804.2

  • Sequence: Human ACE2 (18-740) extracellular domain.

  • Tag: His Tag on its C-Terminus

  • Expression: HEK293 animal free culture

  • MW: 110-120 KD

  • Purity: >95%

  • Endotoxin:< 1.0 EU per μg protein as determined by the LAL method.

  • Biological activity test: EC50= 0.235 nM/L ~1.05 nM/L Specific Binding

  • to the recombinant S-RBD of SARS-CoV-2 (2019-nCoV) coated microplates

  • (200ng/well)Protein ID: NP_068576.1

  • The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH,
    is >1000 pmols/min/μg.